In this study, the effect of fabric density and different colors (black and red) on ultraviolet protection factor of woven fabric investigated. The fabric of different composition (100% cotton, 60/40 CVC), two color (black, red) and various density (Ends per inch, Picks per inch) collected. Then UPF rating was measured by spectrophotometer in vitro method. Experimental result showed that higher the fabric density and weight; higher the protection from ultraviolet rays. Besides, it also revealed that black fabric has more UV protection ability than the red one. Another finding of this study is that polyester content increases the UPF value.